Cas:5132-30-9 BMOE: A Short-Spacer Homobifunctional Crosslinker for Sulfhydryl Mapping
1,2-Bis(maleimido)ethane (BMOE) is a widely used homobifunctional sulfhydryl-reactive crosslinker designed for high-precision protein conjugation and structural biology research. Featuring two highly reactive maleimide groups connected by a short spacer arm, BMOE selectively reacts with thiol groups in cysteine residues, forming stable thioether bonds.
|
Property |
Specification |
|
Product
Name |
1,2-Bis(maleimido)ethane |
|
Abbreviation |
BMOE |
|
CAS Number |
|
|
Molecular Formula |
C10H8N2O4 |
|
Molecular Weight |
220.18 |
|
Purity (HPLC) |
≥ 98% |
|
MDL Number |
MFCD00005517 |
|
Reactive Group |
Maleimide |
|
Spacer Arm |
8.0 Å |
|
Solubility |
Soluble in DMSO or DMF |
Due to its short spacer length of approximately 8 Å, BMOE is particularly valuable in studies requiring accurate mapping of close-range protein–protein interactions. This property makes it an essential reagent in chemical biology, proteomics, structural biology, and drug discovery research.
Finetech Industry Limited supplies high-purity BMOE for research applications, supporting laboratories, biotech companies, and pharmaceutical developers worldwide.
Chemical and Structural Characteristics
BMOE is designed to provide high specificity and controlled crosslinking between sulfhydryl-containing biomolecules.
Key structural features include:
Two maleimide reactive groups targeting thiol groups in proteins
Homobifunctional crosslinker design
Short spacer arm (~8 Å) enabling precise interaction mapping
Stable thioether bond formation
Efficient reaction under mild physiological conditions
The short spacer arm allows researchers to detect protein interactions occurring at very close molecular distances, improving structural interpretation in biochemical studies.
Key Applications of BMOE
1. Protein–Protein Interaction Mapping
One of the most important applications of BMOE crosslinker is the investigation of protein–protein interactions (PPIs).
Because of its short crosslinking distance, BMOE allows scientists to:
Identify direct contact sites between proteins
Confirm protein complex formation
Map interaction interfaces
This approach is commonly used in crosslinking mass spectrometry (XL-MS) experiments.
2. Structural Biology and Protein Conformation Studies
BMOE is widely used in structural biology research to study the spatial organization of proteins.
By crosslinking nearby cysteine residues, researchers can:
Determine distance constraints within protein complexes
Analyze conformational changes in proteins
Support cryo-EM and X-ray crystallography modeling
Short-range crosslinkers like BMOE provide valuable structural data for protein folding and interaction studies.
3. Antibody and Protein Conjugation
BMOE is also used in bioconjugation chemistry to create stable protein conjugates.
Applications include:
Antibody–protein conjugation
Enzyme immobilization
Biosensor development
Targeted labeling of cysteine residues
The thiol-specific reactivity of BMOE allows controlled conjugation without modifying other amino acid residues.
4. Chemical Biology and Drug Discovery Research
In pharmaceutical research, BMOE supports studies such as:
Target validation
Protein complex analysis
Screening interaction inhibitors
Mechanistic studies of signaling pathways
By stabilizing transient protein interactions, BMOE helps researchers better understand drug targets and biological pathways.
Advantages of Using BMOE
Compared with longer spacer crosslinkers, BMOE offers several important advantages:
High spatial precision
The ~8 Å spacer length enables detection of very close molecular interactions.
Selective thiol reactivity
Maleimide groups react specifically with cysteine residues, minimizing non-specific reactions.
Stable crosslink formation
BMOE forms irreversible thioether bonds that remain stable under physiological conditions.
Compatibility with multiple analytical techniques
It is compatible with:
Mass spectrometry
SDS-PAGE analysis
Structural modeling
Proteomics workflows
Finetech Industry Limited – Reliable Supplier of BMOE
Finetech Industry Limited is committed to supplying high-quality research chemicals for laboratories worldwide. Our 1,2-Bis(maleimido)ethane is manufactured under strict quality control to ensure:
High purity
Consistent batch quality
Reliable analytical data
Fast global shipping
We support researchers in chemical biology, proteomics, and pharmaceutical development with dependable reagent supply.
Conclusion
1,2-Bis(maleimido)ethane (BMOE) is a powerful thiol-reactive crosslinking reagent widely used in modern biochemical research. Its short spacer arm, high specificity, and stable crosslinking capability make it an ideal tool for investigating protein interactions, structural biology, and bioconjugation applications.
With reliable quality and professional service, Finetech Industry Limited provides BMOE to support cutting-edge research across the life sciences.