Technical Analysis of 5-(Beta-Hydroxyethyl)-4-Methylthiazole-2-Carboxylic Acid (CAS 75113-60-9 )

5-(Beta-hydroxyethyl)-4-methylthiazole-2-carboxylic acid, also identified by CAS75113-60-9, is a high-value thiazole-based heterocyclic compound. With a molecular formula of C7H9NO3S and a molecular weight of 187.22, this molecule serves as a pivotal intermediate in the biosynthesis of thiamine (Vitamin B1).

Finetech Industry Limited provides high-purity 5-(beta-hydroxyethyl)-4-methylthiazole-2-carboxylic acid to support advanced research in microbial metabolic pathways, enzymology, and pharmaceutical synthesis.


Role in Thiamine (Vitamin B1) Biosynthesis

The biological significance of this compound centers on its role in the thiazole moiety formation during thiamine biosynthesis. In microbial and plant systems, the enzyme-mediated synthesis of thiamine requires specific thiazole precursors.

1.  Pathway Integration: This compound is a key structural analogue in the enzymatic reactions involving THI4 (yeast) or THI1 (plants).

2.  Cofactor Production: It facilitates the condensation with 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP), the precursor to the active cofactor thiamine pyrophosphate (TPP).

3.  Metabolic Engineering: Researchers utilize this intermediate to study sulfur transfer mechanisms and metabolic flux in vitamin biosynthesis.


Key Applications in Research and Industry

1. Enzymology and Mechanism Studies

Due to its hydroxyl and carboxyl functional groups, this molecule is an ideal substrate for examining enzyme active-site interactions. It is widely used to probe the catalytic pathways of thiazole-forming enzymes and sulfur-carrier proteins.

2. Medicinal Chemistry and Drug Discovery

The thiazole scaffold is a "privileged structure" in pharmacology. 5-(Beta-hydroxyethyl)-4-methylthiazole-2-carboxylic acid acts as a versatile building block for:

·         Antimicrobial Agents: Developing novel heterocycles targeting bacterial metabolism.

·         Anti-inflammatory Research: Synthesis of thiazole-based enzyme inhibitors.

·         Ligand Development: Construction of complex heterocyclic ligands for organic catalysis.

3. Microbial Biotechnology

In fermentation optimization and microbial strain engineering, understanding the concentration and regulation of this thiazole intermediate is crucial for maximizing vitamin production yields.


Technical Specifications (Finetech Standard)

Property

Specification

CAS Number

75113-60-9

Molecular Formula

C7H9NO3S

Molecular Weight

187.22

Purity (HPLC)

95.0%

Appearance

Solid

MDL Number

MFCD01311030

Functional Groups

Thiazole ring, Hydroxyl (-OH), Carboxylic acid (-COOH)


Why Choose Finetech Industry Limited?

Finetech Industry Limited is a leading global supplier of specialized research chemicals. We ensure that our CAS 75113-60-9 products meet the most stringent laboratory requirements:

·         High Purity: Guaranteed 95% purity via HPLC for consistent experimental results.

·         Full Documentation: Every batch is provided with a Certificate of Analysis (COA), H-NMR, and SDS.

·         Global Supply Chain: Reliable logistics and flexible packaging options for academic and industrial R&D.


References & Citations

1.  Chatterjee, A., et al. (2011). "The Biosynthesis of Thiamine (Vitamin B1)." Chemical Society Reviews.

2.  Jurgenson, C. T., et al. (2009). "The Structural Biology of Vitamin B1 Biosynthesis." Annual Review of Biochemistry.

3.  Begley, T. P., et al. (1999). "Thiamin Biosynthesis in Prokaryotes." Archives of Microbiology